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d 0015  (Invent Biotechnologies)


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    Invent Biotechnologies d 0015
    D 0015, supplied by Invent Biotechnologies, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d 0015/product/Invent Biotechnologies
    Average 90 stars, based on 3 article reviews
    d 0015 - by Bioz Stars, 2026-02
    90/100 stars

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    bacterial total protein extraction kit  (Invent Biotechnologies)
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    The expression of heat shock proteins and GAS exotoxins by AR-12 treatment. The NZ131 strain was incubated with different concentrations of AR-12 at 37 °C for 2 h, and <t>total</t> <t>protein</t> was isolated by <t>bacterial</t> total protein <t>extraction</t> <t>kit.</t> The protein samples were separated using SDS–PAGE and blotted with anti-DnaK or anti-GroEL antibodies and developed using an ECL Western blot detection kit. Furthermore, the mid-log and late-log phases of the GAS culture supernatants with or without AR-12 treatment were assessed by Western blotting using anti-SPE B and anti-SLO monoclonal antibody, as described in the Materials and Methods Section.
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    The expression of heat shock proteins and GAS exotoxins by AR-12 treatment. The NZ131 strain was incubated with different concentrations of AR-12 at 37 °C for 2 h, and <t>total</t> <t>protein</t> was isolated by <t>bacterial</t> total protein <t>extraction</t> <t>kit.</t> The protein samples were separated using SDS–PAGE and blotted with anti-DnaK or anti-GroEL antibodies and developed using an ECL Western blot detection kit. Furthermore, the mid-log and late-log phases of the GAS culture supernatants with or without AR-12 treatment were assessed by Western blotting using anti-SPE B and anti-SLO monoclonal antibody, as described in the Materials and Methods Section.
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    The expression of heat shock proteins and GAS exotoxins by AR-12 treatment. The NZ131 strain was incubated with different concentrations of AR-12 at 37 °C for 2 h, and total protein was isolated by bacterial total protein extraction kit. The protein samples were separated using SDS–PAGE and blotted with anti-DnaK or anti-GroEL antibodies and developed using an ECL Western blot detection kit. Furthermore, the mid-log and late-log phases of the GAS culture supernatants with or without AR-12 treatment were assessed by Western blotting using anti-SPE B and anti-SLO monoclonal antibody, as described in the Materials and Methods Section.

    Journal: International Journal of Molecular Sciences

    Article Title: AR-12 Has a Bactericidal Activity and a Synergistic Effect with Gentamicin against Group A Streptococcus

    doi: 10.3390/ijms222111617

    Figure Lengend Snippet: The expression of heat shock proteins and GAS exotoxins by AR-12 treatment. The NZ131 strain was incubated with different concentrations of AR-12 at 37 °C for 2 h, and total protein was isolated by bacterial total protein extraction kit. The protein samples were separated using SDS–PAGE and blotted with anti-DnaK or anti-GroEL antibodies and developed using an ECL Western blot detection kit. Furthermore, the mid-log and late-log phases of the GAS culture supernatants with or without AR-12 treatment were assessed by Western blotting using anti-SPE B and anti-SLO monoclonal antibody, as described in the Materials and Methods Section.

    Article Snippet: Total protein contents of each group were isolated using the Bacterial Total Protein Extraction Kit (Invent Biotechnology, Plymouth, MN, USA).

    Techniques: Expressing, Incubation, Isolation, Protein Extraction, SDS Page, Western Blot